tag:blogger.com,1999:blog-4457216402399127579.post7850934810353099878..comments2024-03-13T11:06:06.997-04:00Comments on Next-Gen Sequencing: MiSeq replaces 454 in Microbiome projectsAnonymoushttp://www.blogger.com/profile/14602560263535951430noreply@blogger.comBlogger7125tag:blogger.com,1999:blog-4457216402399127579.post-52421705959424623402022-08-07T04:20:21.663-04:002022-08-07T04:20:21.663-04:00Interesting thouughtsInteresting thouughtsNapa Boilershttps://www.boilers-radiators.com/us/radiators-california/napa-boilers.shtmlnoreply@blogger.comtag:blogger.com,1999:blog-4457216402399127579.post-48900035652272216722021-09-02T11:12:51.251-04:002021-09-02T11:12:51.251-04:00I really want to thank Dr Emu for saving my marria...I really want to thank Dr Emu for saving my marriage. My wife really treated me badly and left home for almost 3 month this got me sick and confused. Then I told my friend about how my wife has changed towards me. Then she told me to contact Dr Emu that he will help me bring back my wife and change her back to a good woman. I never believed in all this but I gave it a try. Dr Emu casted a spell of return of love on her, and my wife came back home for forgiveness and today we are happy again. If you are going through any relationship stress or you want back your Ex or Divorce husband you can contact his whats app +2347012841542 or email emutemple@gmail.com website: Https://emutemple.wordpress.com/<br />Ric Claytonhttps://www.blogger.com/profile/09645806549640826100noreply@blogger.comtag:blogger.com,1999:blog-4457216402399127579.post-43485183556576676902012-09-07T18:15:42.148-04:002012-09-07T18:15:42.148-04:00Bioinformatics students are very important part of...Bioinformatics students are very important part of our societyCustom Lapel Pinshttp://www.maxlapelpins.com/lapelpins.htmlnoreply@blogger.comtag:blogger.com,1999:blog-4457216402399127579.post-86668273529524522302012-08-06T15:19:12.021-04:002012-08-06T15:19:12.021-04:00Curious about your pooling protocol and how much P...Curious about your pooling protocol and how much PhiX to spike with to make this work. Caporaso paper pooled only 24 samples together, but 192 is much better, and 2000 reads is perfectly adequate for most applications. We are soon acquiring a MiSeq in our lab and wish to quickly establish an amplicon sequencing protocol.Andrewhttps://www.blogger.com/profile/16461107374186185973noreply@blogger.comtag:blogger.com,1999:blog-4457216402399127579.post-49067606462995655272012-07-15T18:45:43.661-04:002012-07-15T18:45:43.661-04:00Hi Chris,
The primers are the same as the ones us...Hi Chris,<br /><br />The primers are the same as the ones used in the Caporaso 2012 ISME article. The supplemental material has detailed methods and full primer sequences.<br /><br />http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej20128a.html<br /><br />-LauraAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-4457216402399127579.post-27193057859631360082012-07-02T15:27:10.899-04:002012-07-02T15:27:10.899-04:00+1!!+1!!Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-4457216402399127579.post-38306377622154372402012-07-02T12:22:35.666-04:002012-07-02T12:22:35.666-04:00Hi there,
I would be ecstatically happy if you co...Hi there,<br /><br />I would be ecstatically happy if you could publish the primers used. Have had a poke around the internet and have not yet been able to find them.<br /><br />Thank you in advance,<br /><br />ChrisAnonymousnoreply@blogger.com