Sep 26, 2013

Pig Genome Problems

I have had a few issues with genome annotation in the past couple of weeks that highlight how dependent NGS methods are on these reference files. In a mouse RNA-seq project, we found that RNA reads from some olfactory receptor genes were getting low expression counts (using TopHat/Cufflinks) because RNA reads were not mapping within the known exons of the genes. When we looked at the actual alignment BAM files in IGV, it was clear that the exon boundaries in the reference (RefSeq) genes were wrong. Both the 3' and 5' ends of some genes were missing regions with lots of RNA alignments. It would be nice to make a new reference genome using our RNA-seq reads to create our own annotation, but it is actually quite difficult to get one RNA sample with all genes expressed at useful levels.

In a different RNA-seq project on pig (a model system for cardiology), we ran the standard TopHat/Cufflinks pipeline using the RefSeq gene annotation from the Illumina IGENOMES project (a convenient way to get FASTA, GTF, and Bowtie index files for many genomes: When we looked at the alignment QC using the CollectRnaSeqMetrics from the Picard toolkit, we found that only about 20% of the reads were mapped to exons. A closer look at the annotation file showed that the RefSeq genome for pig (Sus Scrofa) only has about 4,000 genes. That just does not work for RNA-seq. ENSEMBL has a much more complete gene annotation for pig, but the ENSEMBL GTF file is not read nicely by TopHat/Cufflinks.  Thanks to my very helpful friend Igor Dolgalev, I learned that the UCSC Genome Browser has a reformatted ENSEMBL GTF annotation for pig (and most other genomes), but it is missing gene names. The GTF from UCSC Table Browser looks like this:
chr1 susScr3_ensGene stop_codon 268374986 268374988 0 - . gene_id "ENSSSCT00000005917"; transcript_id "ENSSSCT00000005917";
This GTF file has over 30,000 genes and 500,000 lines for individual exons.
UCSC also has a  conversion table for susScr3.ensemblToGeneName with names for about 20,000 of the ENSEMBL genes.  I have not figured out a simple way to write these names back into the GTF file without breaking it, so for now I am matching the names into the final gene expression results.
My only conclusion from these issues with reference genomes is that we depend on these things to be right, and when they are not, NGS data analysis can be badly messed up. There is no simple solution for providing a perfect reference for every genome for every purpose, so bioinformaticians need to stay on their toes and dig deeper when results from standard methods don't make sense.


vidhya said...

Can you explain more on how and why tophat does not read Ensembl gtf file well ?
Thank you


Unknown said...

ENSEMBL uses different chromosome names, so either you start with their version of the genome to build Bowtie index and align BAM files, or change all chromosome names in the GTF. Also, for ENSEMBL GTF files, each genome seems to have unique problems in TopHat. There is a big discussion on SeqAnswers about finding and fixing one overly long transcript that breaks Cufflinks in the mouse genome. In the Pig, there are a bunch of oddly named chromosome fragments.

Anacyte Laboratories said...

Amazing website & awesome article, thanks for sharing with us

RNA Sequencing

Single Cell RNA Sequencing

Protein Stabilization

Ric Clayton said...

I really want to thank Dr Emu for saving my marriage. My wife really treated me badly and left home for almost 3 month this got me sick and confused. Then I told my friend about how my wife has changed towards me. Then she told me to contact Dr Emu that he will help me bring back my wife and change her back to a good woman. I never believed in all this but I gave it a try. Dr Emu casted a spell of return of love on her, and my wife came back home for forgiveness and today we are happy again. If you are going through any relationship stress or you want back your Ex or Divorce husband you can contact his whats app +2347012841542 or email website: Https://